Exploring Experimental Hematology: March 2024 (Volume 131)
G-CSF−induced hematopoietic stem cell mobilization from the embryonic hematopoietic niche does not require neutrophils and macrophages (Ji Wook Kim, Evan A. Fedorova, and Leonard I. Zon) In this issue of Simply Blood, we are highlighting one of the journal’s latest manuscripts by first author Ji Wook Kim. This study was conducted in Dr. Leonard Zon’s laboratory (Department of Stem Cell and Regenerative Biology, Harvard Medical School) and has demonstrated that the Granulocyte colony-stimulating factor (G-CSF) induced hematopoietic stem and progenitor cell (HSPC) mobilization from the embryonic hematopoietic niche in not neutrophil and macrophage depended. The discrepancies in literature suggesting G-CSF either acts directly to mobilize HSPCs or requires myeloid cells has created ambiguity regarding the mechanism of action of G-CSF. Kim et al, developed a stable transgenic line with a heat-inducible csf3b , a zebrafish ortholog of human CSF3 , which translates into G-CSF and confirme