Posts

Message from the President: Emmanuelle Passegué

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Dear ISEH members and stakeholders,

As you are undoubtedly aware, COVID-19 has been making headlines around the world for the past
few weeks and months. On behalf of the ISEH Board of Directors, I would like to extend our heartfelt wishes that you, your family and friends are well. 

Here, at ISEH, we are continuing to monitor the situation closely to determine whether any changes will occur for the ISEH 49th Annual Scientific Meeting in New York City on 20-23 August, 2020. The health and safety of our event attendees and partners is of the utmost importance and we will be following any recommended guidelines and best practices laid out by relevant health and safety entities as August approaches.

In response to the uncertainty we face due to the COVID-19 outbreaks around the world, ISEH will be extending our Call for Abstracts deadline to May 8 (previously April 13). Please note, submission of an abstract is FREE to ISEH members. We hope that you will continue to submit abstracts …

Lab Spotlight: Cristina Lo Celso

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Cristina Lo Celso obtained her PhD exploring epidermal stem cells and the role of beta-catenin signalling in adult epidermal cell fate specification at University College London/Cancer Research UK, under the supervision of Fiona Watt. She then moved to Boston to join David Scadden’s lab as a postdoctoral fellow, where she established novel microscopy techniques and tools to visualise haematopoietic stem cells in the bone marrow microenvironment for the first time in vivo and in real-time. Cristina is currently a Professor of stem cell biology at Imperial College London and a satellite investigator at the Francis Crick Institute, where her research focuses on understanding haematopoietic stem cell function during steady-state and in the presence of stress, such as leukaemia development and infection. 

How long have you had your lab?
10 years

How many members make up your lab?
We are 8 in total – 3 postdocs and 5 PhD students. I also co-supervise a number of PhD students who attend la…

Live-animal imaging of native haematopoietic stem and progenitor cells: An interview with Constantina Christodoulou, Joel Spencer and Shu-Chi (Allison) Yeh

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Over the last few decades, hematopoietic stem cells (HSCs) have been prospectively identified and studied based on their ability to functionally regenerate and maintain the entire blood system through serial transplantation.  While many important determinants of HSC activity have been elucidated through these foundational assays, less is known as to how HSCs function in unperturbed states within their native microenvironments.  Advancements in genetic tools have recently begun to open up opportunities for researchers to address these gaps in our understanding of HSC biology.  Here, I interviewed co-first authors Dr. Constantina Christodoulou, Dr. Joel Spencer and Dr. Shu-Chi (Allison) Yeh, who provided some further commentary on their recent successful efforts to image HSCs in live animals with the support of collaborative teams led by Dr. Fernando Camargo and Dr. Charles Lin (Christodoulou et al., 2020).

1.    First off, congratulations on your monumental achievement of imaging HSCs …

The Best Part of a Cake is the Frosting; How to Choose Service Obligations that Enhance Your Career

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Scientists are like bakers. Although a great baker will work hard and put all their effort to make an amazing cake, it would remain incomplete and less attractive without frosting. The same could be applied to your career in biomedical research. But what is the perfect ratio of frosting for showcasing your cake?


A prolific scientific career is not all about the science. Although you must invest countless hours to diversify your expertise, make yourself attractive for future employers and collaborators, and progress throughout your journey as a scientist, there are many skills which are essential for becoming a successful independent researcher that you cannot learn in the lab. In many institutions, including scientists holding a tenure-track position in the United States, there are requirements above scholarly activity such as teaching and service components. To this end, as careers evolve and expand, many scientists take on additional service and leadership roles within their institu…

Project Management Part 2: Developing a Project Timeline

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Imagine you’ve got a two-year grant to study a particular hematologic phenotype, or you are a PhD student whose project has just gained traction, but your remaining time is limited by your program. You know generally what experiments you want to do, so isn’t that enough?

Sure, if you want to increase the risk of not completing your project on time and within budget.

All of us know that science is not the same as building a bridge; there’s a higher level of serendipity involved wherein an experiment can negate the hypothesis or open an unexpected avenue to be pursued. However, developing a strategy that orders experiments logically and in a way that fully takes advantage of the time available can ensure efficiency regardless of the scientific outcome. Below are some steps to take to help devise a timeline for your project:
Revisit the project scope. In the first part of this series we discussed the scope of the project; i.e., what the project involves. This is defined based on what work…

Lab Spotlight: Bonifer Lab

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Each month, Simply Blood spotlights a lab focused on the research of basic hematology, immunology, stem cell research, cell and gene therapy, and other related aspects. Get to know these different labs around the world! This month, we are featuring the Bonifer Lab at the University of Birmingham in Birmingham, UK.

How long have you had your lab?
I have been an independent investigator since 1990, always in the field of gene regulation. First at the University of Freiburg in Germany, then at the University of Leeds, UK. In 2011 we moved to the University of Birmingham, UK

How many members make up your lab?  Students/postdocs?
We are currently 5 students, 6 postdocs and two technicians.

What is the major research theme of your lab?
Our major research theme is to understand how the gene regulatory machinery and outside signals program a transcriptionally active chromatin structure and drive cell fate decisions in the hematopoietic system. We are interested both in normal, but also aberrant …

Exploring Experimental Hematology: CD34 and EPCR coordinately enrich functional murine hematopoietic stem cells under normal and inflammatory conditions

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In this issue of Simply Blood, Grant Challen is exploring Experimental Hematology by highlighting and deconstructing one of his favorite manuscripts from the ISEH society journal: "CD34 and EPCR coordinately enrich functional murine hematopoietic stem cells under normal and inflammatory conditions."



Exp Hematol. 2019 Dec 18.

Rabe JL, Hernandez G, Chavez JS, Mills TS, Nerlov C, Pietras EM.

In a recent study in the laboratory of Dr. Eric Pietras (University of Colorado), Rabe et al use the cell surface markers EPCR and CD34 to coordinately distinguish HSCs with distinct functional and molecular properties within the SLAM (Lineage- c-Kit+ Sca-1+ CD48- CD150+) gate under conditions of chronic inflammation.  By giving wild-type mice a 20-day treatment course of the pro-inflammatory cytokine IL-1, the authors show that the EPCR+ CD34- cells within the SLAM gate are largely unaffected.  The numbers of EPCR+ CD34- HSCs does not dramatically change under chronic inflammation mediated…