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Exploring Experimental Hematology: CD34 and EPCR coordinately enrich functional murine hematopoietic stem cells under normal and inflammatory conditions

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In this issue of Simply Blood, Grant Challen is exploring Experimental Hematology by highlighting and deconstructing one of his favorite manuscripts from the ISEH society journal: "CD34 and EPCR coordinately enrich functional murine hematopoietic stem cells under normal and inflammatory conditions."



Exp Hematol. 2019 Dec 18.

Rabe JL, Hernandez G, Chavez JS, Mills TS, Nerlov C, Pietras EM.

In a recent study in the laboratory of Dr. Eric Pietras (University of Colorado), Rabe et al use the cell surface markers EPCR and CD34 to coordinately distinguish HSCs with distinct functional and molecular properties within the SLAM (Lineage- c-Kit+ Sca-1+ CD48- CD150+) gate under conditions of chronic inflammation.  By giving wild-type mice a 20-day treatment course of the pro-inflammatory cytokine IL-1, the authors show that the EPCR+ CD34- cells within the SLAM gate are largely unaffected.  The numbers of EPCR+ CD34- HSCs does not dramatically change under chronic inflammation mediated…

Lab Spotlight: Kousteni Lab

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Each month, Simply Blood spotlights a lab focused on the research of basic hematology, immunology, stem cell research, cell and gene therapy, and other related aspects. Get to know these different labs around the world! This month, we are featuring the Kousteni Lab at Columbia University in New York, USA.


1. How long have you had your lab? 
I started my lab when I moved to Columbia University in July of 2006.

2. How many members make up your lab?  Students/postdocs?
Currently the lab consists of 1 technician/lab-manager, 2 postdocs, 4 associate research scientists and one assistant professor. The summer is our busiest time of the year since we host undergraduate trainees from the SPURS program of the Department of Physiology and Cellular Biophysics for underrepresented students, a teacher trainee from Columbia’s Summer Research Program for Science Teachers, undergraduates from NYIT and visiting scientists or postdocs from various Universities.

3. What is the major research theme of…