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Showing posts from February, 2020

Exploring Experimental Hematology: CD34 and EPCR coordinately enrich functional murine hematopoietic stem cells under normal and inflammatory conditions

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In this issue of Simply Blood, Grant Challen is exploring Experimental Hematology by highlighting and deconstructing one of his favorite manuscripts from the ISEH society journal: "CD34 and EPCR coordinately enrich functional murine hematopoietic stem cells under normal and inflammatory conditions."



Exp Hematol. 2019 Dec 18.

Rabe JL, Hernandez G, Chavez JS, Mills TS, Nerlov C, Pietras EM.

In a recent study in the laboratory of Dr. Eric Pietras (University of Colorado), Rabe et al use the cell surface markers EPCR and CD34 to coordinately distinguish HSCs with distinct functional and molecular properties within the SLAM (Lineage- c-Kit+ Sca-1+ CD48- CD150+) gate under conditions of chronic inflammation.  By giving wild-type mice a 20-day treatment course of the pro-inflammatory cytokine IL-1, the authors show that the EPCR+ CD34- cells within the SLAM gate are largely unaffected.  The numbers of EPCR+ CD34- HSCs does not dramatically change under chronic inflammation mediated…